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Part:BBa_K4212020

Designed by: Yuancheng Ding   Group: iGEM22_Imperial_College_London   (2022-09-30)


0A-Spacer

Neutral spacer sequence that separates the coding sequences of Cas9 and D15 , and constructs pCas9 level 1 plasmid

Figure 1. The schematic graphic of 0A-Spacer

References

[1] Garrett, S.C. (2021) Pruning and Tending Immune Memories: Spacer Dynamics in the CRISPR Array. Frontiers in Microbiology. 12. https://www.frontiersin.org/articles/10.3389/fmicb.2021.664299.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None