RBS

Part:BBa_K4212019

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)


Optimal_RBS

Very strong RBS in B. subtilis, increases expression to 25 folds.

Usage and Biology

Ribosome binds to this place and start translation. Optimal RBS can improve the translation efficiency and protein expression level.

References

[1] Nowroozi, F.F., Baidoo, E.E.K., Ermakov, S., Redding-Johanson, A.M., Batth, T.S., Petzold, C.J. & Keasling, J.D. (2014) Metabolic pathway optimization using ribosome binding site variants and combinatorial gene assembly. Applied Microbiology and Biotechnology. 98 (4), 1567–1581. doi:10.1007/s00253-013-5361-4.

[2] McLaughlin, J.R., Murray, C.L. & Rabinowitz, J.C. (1981) Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene. Journal of Biological Chemistry. 256 (21), 11283–11291. doi:10.1016/S0021-9258(19)68589-3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None