RBS

Part:BBa_K4212017

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)


RBS2

Strong RBS in B. subtilis .

Usage and Biology

Ribosome binds to this place and start translation.

Figure 1. The schematic graphic of RBS2

References

[1] Vellanoweth, R.L. & Rabinowitz, J.C. (1992) The influence of ribosome-binding-site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Molecular Microbiology. 6 (9), 1105–1114. doi:10.1111/j.1365-2958.1992.tb01548.x.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 6
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None