Regulatory

Part:BBa_K4212015

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)


PsspB

Potential promoter regulating sspB expression in B. subtilis. PsspB only express strongly after the initiation of sporulation.

Usage and Biology

Use for transcription of B.subtilis gene.

Figure 1. The schematic graphic of PsspB promoter

References

[1] Cabrera-Martinez, R.-M., Tovar-Rojo, F., Vepachedu, V.R. & Setlow, P. (2003) Effects of Overexpression of Nutrient Receptors on Germination of Spores of Bacillus subtilis. Journal of Bacteriology. 185 (8), 2457–2464. doi:10.1128/JB.185.8.2457-2464.2003. [2] Stewart, K.-A.V., Yi, X., Ghosh, S. & Setlow, P. (2012) Germination Protein Levels and Rates of Germination of Spores of Bacillus subtilis with Overexpressed or Deleted Genes Encoding Germination Proteins. Journal of Bacteriology. 194 (12), 3156–3164. doi:10.1128/JB.00405-12.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 66
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None