Regulatory

Part:BBa_K4212014

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)


PlepA

Strong constitutive promoter in B. subtilis .

Usage and Biology

Responsible for the transcription of B. subtilis gene.

Figure 1. The schematic graphic of PlepA

References

[1] Tran, D.T.M., Phan, T.T.P., Huynh, T.K., Dang, N.T.K., Huynh, P.T.K., Nguyen, T.M., Truong, T.T.T., Tran, T.L., Schumann, W. & Nguyen, H.D. (2017) Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis. Microbial Cell Factories. 16 (1), 130. doi:10.1186/s12934-017-0747-0.

[2] Lu, Z., Yang, S., Yuan, X., Shi, Y., Ouyang, L., Jiang, S., Yi, L. & Zhang, G. (2019) CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis. Nucleic Acids Research. 47 (7), e40. doi:10.1093/nar/gkz072.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None