Regulatory

Part:BBa_K4212013

Designed by: Youzi Bi   Group: iGEM22_Imperial_College_London   (2022-09-30)


Hyperspank

Inducible promoter with IPTG.

Usage and Biology

Use for B.subtilis gene transcription.

Figure 1. The schematic graphic of Hyperspank promoter

References

[1] Vavrová, Ľ., Muchová, K. & Barák, I. (2010) Comparison of different Bacillus subtilis expression systems. Research in Microbiology. 161 (9), 791–797. doi:10.1016/j.resmic.2010.09.004.

[2] Nordholt, N., van Heerden, J., Kort, R. & Bruggeman, F.J. (2017) Effects of growth rate and promoter activity on single-cell protein expression. Scientific Reports. 7 (1), 6299. doi:10.1038/s41598-017-05871-3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None