Coding

Part:BBa_K4212008

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)


GerA_operon

Operon encoding the GerA germinant receptor of B. subtilis. Can be used to introduce the receptor in knockout strains without germinant receptors, as well as for directed evolution of said receptor using saturation mutagenesis or error-prone PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3506
    Illegal BglII site found at 3038
    Illegal XhoI site found at 628
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3506
    Illegal PstI site found at 521
    Illegal PstI site found at 3457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2693


[edit]
Categories
Parameters
None