Coding
VP64
Part:BBa_K4211013
Designed by: Peisong Wu Group: iGEM22_BNUZH-China (2022-09-04)
VP64: The herpes simplex virus-derived VP16 activation domain
VP64, the herpes simplex virus-derived VP16 activation domain, is a kind of transcriptional activators. It is fused to FHY1. When illuminated with the 660 nm light, PhyA can bind with FHY1 and can be transported into the nucleus. ΔPhyA–Gal4/FHY1–VP64 combination demonstrated the best switching performance than any other.
Usage and Biology
We used it as a transcriptional activator to activate the transcription of MazF (BBa_K302033).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |