Coding
VP64

Part:BBa_K4211013

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-09-04)


VP64: The herpes simplex virus-derived VP16 activation domain

VP64, the herpes simplex virus-derived VP16 activation domain, is a kind of transcriptional activators. It is fused to FHY1. When illuminated with the 660 nm light, PhyA can bind with FHY1 and can be transported into the nucleus. ΔPhyA–Gal4/FHY1–VP64 combination demonstrated the best switching performance than any other.


Usage and Biology

We used it as a transcriptional activator to activate the transcription of MazF (BBa_K302033).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None