Part:BBa_K4211010
P2A
P2A is a porcine teschovirus-1 derived self-cleavage 2A peptide. Compared to other 2A self-cleaving peptides, P2A has been shown to have relatively higher efficiency. It can cause co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A linker works by ribosome skipping mechanism, that is, while translation is taking place, the ribosome skips an amino acid at the P2A linker site, thereby effectively separating the upstream protein from the downstream protein. Due to these abilities, 2A Linkers can be effectively used as a small size replacement for IRES (Internal Ribosomal Entry Sites) while designing DNA sequences for recombinant protein (non-fused) in both prokaryotic and eukaryotic systems. Hence, this is a great tool for synthetic biology.
Usage and Biology
We used P2A to link Gal4 (BBa_K4211009) with FHY1 (BBa_K4211011), thereby effectively separating the upstream protein: Gal4 from the downstream protein: FHY1.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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