Part:BBa_K4211007

PhyA: Red/Far-red light-responsive photoreceptor

In land plants, the ability to sense and respond to red/far-red light depends on proteins of the phytochrome photoreceptor family. PhyA is a well-characterized member of this family that binds covalently to a chromophore known as Phycocyanobilin (PCB). PhyA is a red/far-red light-responsive photoreceptor from the plant Arabidopsis thaliana. The PCB binds covalent to PhyA to produce responses to red light (λ_max = 660 nm) and far-red light (λ_max = 730 nm). Following activation by red light, PhyA is transported into the nucleus based on interactions with shuttle proteins, including FHY1 and FHY1-like (FHL).

Usage and Biology

We used PCB to bind with PhyA to response to red light (λ_max = 660 nm). We would link PhyA with Gal4 (BBa_K4211009). That would bind to the yeast upstream activation sequence of 5×UAS (BBa_K4211005) under red light irradiation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]