Tag
V5
Part:BBa_K4211006
Designed by: Peisong Wu Group: iGEM22_BNUZH-China (2022-08-19)
V5-tag
The V5-tag is a polypeptide isolated from the P and V proteins of simian virus 5 (SV5), consisting of residues 95 to 108, and the amino acid sequence is GKPIPNPLLGLDST. V5-tag is often fused to the N-terminal or C-terminal of target protein for analysis, identification and purification of target protein.
Usage and Biology
We fused it to the C-terminal of MazF (BBa_ K302033) protein for analysis, identification and purification. In order to terminate the transcription, we added TAA sequence to the C-terminal of V5-tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/part_collection
biology | Simian virus 5 |
protein | V5 |