Coding
5×UAS-P(hC

Part:BBa_K4211005

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-08-18)


5xUAS-P(hCMVmin)

5×UAS-P(hCMVmin) contains five tandem repeats of the yeast upstream activating sequence (UAS; Gal4 binding site) upstream of a minimal human cytomegalovirus (CMV) immediate early promoter controlling expression of the downstream target genes.

Usage and Biology

We used 5×UAS-P(hCMVmin) to bind Gal4 and initiate the expression of downstream target genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection
Parameters
biologyYeast