Part:BBa_K4211000
EGF: Human epidermal growth factor
Epidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is 6-kDa and has 53 amino acid residues and three intramolecular disulfide bonds. The EGF gene we selected for our experiment is NM_001178130.3 (Homo sapiens epidermal growth factor (EGF), transcript variant 2). It has a molecular weight of 23.1 kDa. We used EGF to promote the growth of epidermal cells and fibroblasts. The EGF gene was ligated on the adeno-associated virus pBEK plasmid (gift of Y. Liang, Beijing Normal University) used in our study. In application, the IL-2 Signal Sequence (BBa_K4211002) can be fused at the N-terminal of the EGF gene to promote EGF secretion out of the cell.
Figure 1: Western Blot of EGF. The arrow marks the EGF band with a molecular weight of 23.1 kDa.
Figure 2: Construct design: The EGF gene. The EGF gene was inserted after the CMV Promoter.
Usage and Biology
We used EGF and bFGF (BBa_K4211001) to stimulate the growth of epidermal cells and fibroblasts.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| biology | Human |
| protein | EGF |