Composite

Part:BBa_K4209014

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


constitutive FitG

FitG is a transcriptional factor naturally produced by Pseudomonas protegens. The fitG gene is part of a virulence operon which is regulated by three regulatory proteins, including an activator encoded by the fitG gene and a type I secretion system. FitG is an activator of the FitD toxin that has been shown to have insecticidal activity, by inducing the apoptosis of the midgut epithelium as well as paralyzing and destroying insect phagocytes (M et al., 2013) This composite part has a EM7 promoter which is constitutively active, meaning that the plasmid doesn't need to be induced with IPTG to produce the toxin.

Sequence and Features


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 57
    Illegal PstI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 57
    Illegal PstI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 57
    Illegal BglII site found at 86
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 57
    Illegal PstI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 57
    Illegal PstI site found at 117
    Illegal NgoMIV site found at 681
    Illegal AgeI site found at 527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 875


[edit]
Categories
Parameters
None