Composite

Part:BBa_K4209013

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


constitutive FitD

FitD is a toxin naturally produced by Pseudomonas protegens. The fitD gene is part of a virulence operon which is regulated by three regulatory proteins, including an activator encoded by the fitG gene and a type I secretion system. The FitD toxin has been shown to have insecticidal activity, by inducing the apoptosis of the midgut epithelium as well as paralyzing and destroying insect phagocytes (M et al., 2013). Furthermore FitD is also known for its potential as a molluscicidal agent. This composite part has a EM7 promoter which is constitutively active, meaning that the plasmid doesn't need to be induced with IPTG to produce the toxin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
    Illegal NotI site found at 8760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal XhoI site found at 6183
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
    Illegal NgoMIV site found at 506
    Illegal NgoMIV site found at 850
    Illegal NgoMIV site found at 1543
    Illegal NgoMIV site found at 2039
    Illegal NgoMIV site found at 2829
    Illegal NgoMIV site found at 2945
    Illegal NgoMIV site found at 3053
    Illegal AgeI site found at 3788
    Illegal AgeI site found at 5937
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 281
    Illegal BsaI site found at 1148
    Illegal BsaI.rc site found at 1470
    Illegal BsaI.rc site found at 4122


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