Composite

Part:BBa_K4209012

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


fitG inducible

FitG is a transcriptional factor naturally produced by Pseudomonas protegens. The fitG gene is part of a virulence operon which is regulated by three regulatory proteins, including an activator encoded by the fitG gene and a type I secretion system. FitG is an activator of the FitD toxin that has been shown to have insecticidal activity, by inducing the apoptosis of the midgut epithelium as well as paralyzing and destroying insect phagocytes (M et al., 2013). This composite part has an Trc promoter which is inducible with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 39
    Illegal PstI site found at 99
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 39
    Illegal PstI site found at 99
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 39
    Illegal BglII site found at 68
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 39
    Illegal PstI site found at 99
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 39
    Illegal PstI site found at 99
    Illegal NgoMIV site found at 663
    Illegal AgeI site found at 509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 857


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Categories
Parameters
None