Composite

Part:BBa_K4209010

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


cysPUWA_cysDNCQ

This composite part is essential for the uptake and transport of sulfate among the cell. The sulfate is essential for the transformation of pHCA into zosteric acid. cysPUWA allows entry of a sulphate group into the cell, while the cysDNCQ complex is needed for the integration of the sulphate into pHCA (with the help of SULT1A1). (Figure 1)

Pathway_simplified_UNIL2022.jpg

Figure 1. Schematic representation of the ZA production pathway The Tal-fjo enzyme converts the tyrosine, naturally present in the bacteria, into pHCA. CysP or CysPUWA are responsible for the uptake of sulphate into the cell. The CysDNC enzymes replace two phosphate groups of ATP by the sulphate. The sulphate carrier is used by SULT1A1 to incorporate sulphate into coumaric acid creating zosteric acid. CysQ molecule is essential to recycle ATP and allow for a second cycle to start.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 772
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 6147
    Illegal EcoRI site found at 7160
    Illegal PstI site found at 3480
    Illegal PstI site found at 7509
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 772
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 6147
    Illegal EcoRI site found at 7160
    Illegal NheI site found at 7620
    Illegal PstI site found at 3480
    Illegal PstI site found at 7509
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 772
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 6147
    Illegal EcoRI site found at 7160
    Illegal BglII site found at 6805
    Illegal BamHI site found at 1303
    Illegal BamHI site found at 4372
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 772
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 6147
    Illegal EcoRI site found at 7160
    Illegal PstI site found at 3480
    Illegal PstI site found at 7509
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 772
    Illegal EcoRI site found at 3974
    Illegal EcoRI site found at 6147
    Illegal EcoRI site found at 7160
    Illegal PstI site found at 3480
    Illegal PstI site found at 7509
    Illegal AgeI site found at 2119
    Illegal AgeI site found at 4661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7407


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Categories
Parameters
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