Composite

Part:BBa_K4209009

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


cysP_cysDNCQ

This composite part is essential for the uptake and transport of sulfate among the cell. The sulfate is essential for the transformation of pHCA into zosteric acid. cysP allows entry of a sulphate group into the cell, while the cysDNCQ complex is needed for the integration of the sulphate into pHCA (with the help of SULT1A1). (Figure 1)

Pathway_simplified_UNIL2022.jpg

Figure 1. Schematic representation of the ZA production pathway The Tal-fjo enzyme converts the tyrosine, naturally present in the bacteria, into pHCA. CysP or CysPUWA are responsible for the uptake of sulphate into the cell. The CysDNC enzymes replace two phosphate groups of ATP by the sulphate. The sulphate carrier is used by SULT1A1 to incorporate sulphate into coumaric acid creating zosteric acid. CysQ molecule is essential to recycle ATP and allow for a second cycle to start.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1227
    Illegal EcoRI site found at 3400
    Illegal EcoRI site found at 4413
    Illegal PstI site found at 101
    Illegal PstI site found at 4762
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1227
    Illegal EcoRI site found at 3400
    Illegal EcoRI site found at 4413
    Illegal NheI site found at 4873
    Illegal PstI site found at 101
    Illegal PstI site found at 4762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1227
    Illegal EcoRI site found at 3400
    Illegal EcoRI site found at 4413
    Illegal BglII site found at 4058
    Illegal BamHI site found at 1625
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1227
    Illegal EcoRI site found at 3400
    Illegal EcoRI site found at 4413
    Illegal PstI site found at 101
    Illegal PstI site found at 4762
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1227
    Illegal EcoRI site found at 3400
    Illegal EcoRI site found at 4413
    Illegal PstI site found at 101
    Illegal PstI site found at 4762
    Illegal NgoMIV site found at 577
    Illegal NgoMIV site found at 695
    Illegal AgeI site found at 1914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4660


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