Coding

Part:BBa_K4209006

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


fitG gene encoding for the promoter of the FitD toxin

FitG is a transcriptional factor naturally produced by Pseudomonas protegens. The fitG gene is part of a virulence operon which is regulated by three regulatory proteins, including an activator encoded by the fitG gene and a type I secretion system. FitG is an activator of the FitD toxin that has been shown to have insecticidal activity, by inducing the apoptosis of the midgut epithelium as well as paralyzing and destroying insect phagocytes (M et al., 2013).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 46
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 46
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 15
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 46
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 46
    Illegal NgoMIV site found at 610
    Illegal AgeI site found at 456
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 804


[edit]
Categories
Parameters
None