Coding

Part:BBa_K4209005

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


fitD gene encoding for the FitD toxin


FitD is a toxin naturally produced by Pseudomonas protegens. The fitD gene is part of a virulence operon which is regulated by three regulatory proteins, including an activator encoded by the fitG gene and a type I secretion system. The FitD toxin has been shown to have insecticidal activity, by inducing the apoptosis of the midgut epithelium as well as paralyzing and destroying insect phagocytes (M et al., 2013). Furthermore FitD is also known for its potential as a molluscicidal agent. Profile


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5035
    Illegal PstI site found at 493
    Illegal PstI site found at 547
    Illegal PstI site found at 1750
    Illegal PstI site found at 2536
    Illegal PstI site found at 7780
    Illegal PstI site found at 8335
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5035
    Illegal PstI site found at 493
    Illegal PstI site found at 547
    Illegal PstI site found at 1750
    Illegal PstI site found at 2536
    Illegal PstI site found at 7780
    Illegal PstI site found at 8335
    Illegal NotI site found at 8689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5035
    Illegal XhoI site found at 6112
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5035
    Illegal PstI site found at 493
    Illegal PstI site found at 547
    Illegal PstI site found at 1750
    Illegal PstI site found at 2536
    Illegal PstI site found at 7780
    Illegal PstI site found at 8335
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5035
    Illegal PstI site found at 493
    Illegal PstI site found at 547
    Illegal PstI site found at 1750
    Illegal PstI site found at 2536
    Illegal PstI site found at 7780
    Illegal PstI site found at 8335
    Illegal NgoMIV site found at 435
    Illegal NgoMIV site found at 779
    Illegal NgoMIV site found at 1472
    Illegal NgoMIV site found at 1968
    Illegal NgoMIV site found at 2758
    Illegal NgoMIV site found at 2874
    Illegal NgoMIV site found at 2982
    Illegal AgeI site found at 3717
    Illegal AgeI site found at 5866
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 210
    Illegal BsaI site found at 1077
    Illegal BsaI.rc site found at 1399
    Illegal BsaI.rc site found at 4051


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