Coding

Part:BBa_K4209002

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


cysDNCQ

This part encodes for 3 different enzymes: cysDN, cysC and cysQ. cysDN encodes two proteins that create a sulfate activation complex, cysC that catalyzes the synthesis of activated sulfate and cysQ recycles the carrier molecule so that it can accept a new sulfate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 690
    Illegal EcoRI site found at 2863
    Illegal EcoRI site found at 3391
    Illegal PstI site found at 3042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 690
    Illegal EcoRI site found at 2863
    Illegal EcoRI site found at 3391
    Illegal PstI site found at 3042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 690
    Illegal EcoRI site found at 2863
    Illegal EcoRI site found at 3391
    Illegal BglII site found at 32
    Illegal BamHI site found at 2465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 690
    Illegal EcoRI site found at 2863
    Illegal EcoRI site found at 3391
    Illegal PstI site found at 3042
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 690
    Illegal EcoRI site found at 2863
    Illegal EcoRI site found at 3391
    Illegal PstI site found at 3042
    Illegal AgeI site found at 2176
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3143


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Parameters
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