Coding

Part:BBa_K4208002

Designed by: Ferran Agulló i Manobens   Group: iGEM22_Tuebingen   (2022-09-30)


PctD HAMP EnvZ Hybrid


Usage and Biology

This basic part has been developed by team Tuebingen 2022 [1] to sense acetylcholine and choline (and potentially Anatoxin A) with a Hybrid receptor encoding for the PctD Sensing and HAMP Domain and the EnvZ Histidine Kinase/Phosphatase domain.

Results

We successfully cloned this circuit but we did not further testing


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 40
    Illegal NgoMIV site found at 94
    Illegal NgoMIV site found at 844
    Illegal NgoMIV site found at 1090
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None