Regulatory

Part:BBa_K4205002

Designed by: Xinyuan Wang   Group: iGEM22_NJMU-China   (2022-09-04)


PpbrA-PmerR

PpbrA is a lead-specific promoter and PmerR is a mercury-specific promoter, and we constructed a OR gate by concatenating these two promoters. Either lead or mercury can turn on the expression of downstream genes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None