Regulatory

Part:BBa_K4204015

Designed by: Jianfei Song   Group: iGEM22_BNDS_China   (2022-10-08)


pJEx-D + RBS of crystal violet induction system

This part contains pJEx-D promoter (a promoter with a crystal violet regulator) and the original RBS used in the crystal violet induction system. This part should be used with eilR (BBa_K4204004), which codes for the regulatory protein (EilR) of the crystal violet induction system. When crystal violet is absent, EilR will bind to eilOc and inhibit the translation of the downstream gene. If crystal violet is present, it will primarily bind to EilR and change its structure to disable it to bind to eilOc.

For detailed characterization, see BBa_K4204016

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 89
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None