Part:BBa_K4204005
Toehold Switch of the ProQC System
Description
The ProQC system is a synthetic protein quality control system that mimics a eukaryotic translation system to improve protein quality. It only allows translation of full-length mRNA, and thus can improve the quality of full-length protein synthesis in bacteria by avoiding mRNA truncation, reducing the possibility of ribosome trapping, and preventing synthesis of the truncated proteins. This synthetic expression cassette is composed of a toehold switch at the 5’ end of an mRNA and a corresponding complement trigger sequence at the 3’ end of the mRNA. The complementarity between the switch and the trigger enables translation of bacterial mRNA to delay until the 3’ and 5’ ends of the mRNA are linked and circulated by sequence-specific RNA-RNA hybridization, while ribosome can’t bind to the mRNA and initiate translation when the 3’ end of the mRNA hasn’t be transcribed. The toehold switch is the lock of the translation of bacterial mRNA.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 96
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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