Plasmid
ADH1p-DmNa

Part:BBa_K4203002

Designed by: Yixuan Wang ;Zongjun Lee   Group: iGEM22_BJWZ-China   (2022-10-12)


After codon optimization, those part is suitable for the fusion protein ADH1p-DmNan-P2A-mCherry expr

After codon optimization, the fusion protein ADH1p-DmNan-P2A-mCherry wasplaning to express in Saccharomyces cerevisiae BY4741. It was linked to the vector pESC-HIS by adding BamH I and Sac I restriction sites at the 5 'and 3' ends, respectively. 5564-5960 bp represents the ADH1 promoter, 3059-5557 bp part represents DmNan component, 2993-3094 bp represents P2A linker, 2264-2980 bp represents mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3181
    Illegal XbaI site found at 3893
    Illegal XbaI site found at 4886
    Illegal XbaI site found at 9224
    Illegal PstI site found at 1183
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3181
    Illegal NheI site found at 1008
    Illegal NheI site found at 6053
    Illegal PstI site found at 1183
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3181
    Illegal BglII site found at 899
    Illegal BglII site found at 959
    Illegal BglII site found at 3320
    Illegal BamHI site found at 5961
    Illegal XhoI site found at 6031
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3181
    Illegal XbaI site found at 3893
    Illegal XbaI site found at 4886
    Illegal XbaI site found at 9224
    Illegal PstI site found at 1183
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3181
    Illegal XbaI site found at 3893
    Illegal XbaI site found at 4886
    Illegal XbaI site found at 9224
    Illegal PstI site found at 1183
    Illegal NgoMIV site found at 1748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4269
    Illegal BsaI.rc site found at 7394
    Illegal SapI site found at 3252
    Illegal SapI site found at 6311


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Categories
Parameters
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