Measurement

Part:BBa_K4202038

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-10-03)


Pveg-2x leader RNA-spoVG RBS-firefly luciferase for Bacillus subtilis

The firefly luciferase gene is ligated to Pveg-2x leader RNA-spoVG RBS downstream. Different sucrose concentrations were used to induce promoters. This device is used to characterize the promoter Pveg-2x leader RNA-spoVG RBS. The reporter gene firefly luciferase is connected to the downstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1233
    Illegal AgeI site found at 1345
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None