Measurement
Part:BBa_K4202033
Designed by: Zhijian Yan Group: iGEM22_ZJU-China (2022-10-03)
Pveg-1x leader RNA-spoVG RBS-GFP for Bacillus subtilis
The GFP gene is ligated to Pveg-1x leader RNA-spoVG RBS downstream. Different sucrose concentrations were used to induce promoters. This device is used to characterize the promoter Pveg-1x leader RNA-spoVG RBS. The reporter gene GFP is connected to the downstream of the promoter.
Usage and Biology
Usage
This is the measurement device of BBa_K4202025.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1111
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Categories
Parameters
None |