Measurement

Part:BBa_K4202031

Designed by: YIBING HAN   Group: iGEM22_ZJU-China   (2022-10-03)


PsacB-spoVG RBS-firefly luciferase for Bacillus subtilis

The firefly luciferase gene is ligated to PsacB promoter and spoVG RBS downstream. Different sucrose concentrations were used to induce promoters. This device is used to characterize the promoter PsacB-spoVG RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1022
    Illegal AgeI site found at 1134
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None