Measurement

Part:BBa_K4202029

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-10-03)


PsacB-spoVG RBS-GFP for Bacillus subtilis

The GFP gene is ligated to PsacB promoter and spoVG RBS downstream. Different sucrose concentrations were used to induce promoters. This device is used to characterize the promoter PsacB-spoVG RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1110


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Categories
Parameters
None