DNA

Part:BBa_K4202019

Designed by: Zhijian Yan   Group: iGEM22_ZJU-China   (2022-10-03)


leader RNA of PsacB

This sequence is located in the +1-+191 region of the PsacB promoter, encoded both a ribonucleic antiterminator sequence which can bind to the sucrose activated antiterminator protein SacY and a Rho independent transcription terminator. In the absence of sucrose-activated SacY, the inverted repeated sequence of the leader RNA will spontaneously form a hairpin loop structure upstream of the U rich region, resulting in early termination(transcription attenuation); in the presence of sucrose-activated SacY, the hairpin loop structure will be stabilized by the SacY-sucrose complex, which allowing transcription of the downstream gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None