Plasmid

Part:BBa_K4202018

Designed by: ZIHANG YE   Group: iGEM22_ZJU-China   (2022-10-01)


This is a expression vector for the protein expression in Bacillus subtilis.

We connect the P43 promotor(BBa_K2029000) and double terminator(BBa_B0010 and BBa_B0012) to the PHY300PLK(BBa_K4202016). We can utilize this part for the protein expression in Bacillus subtilis. Because we adapt the infusion strategy to construct the plasmid, we delete the MCS and use the same method to insert the CDS.

It`s worth to note that this vector can also be used for the protein expression in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 530
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 530
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 530
    Illegal BglII site found at 11
    Illegal BamHI site found at 339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 530
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 530
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 4323
    Illegal AgeI site found at 5202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3080
    Illegal BsaI site found at 4497


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