Part:BBa_K4202016

PHY-300PLK is a Escherichia coli-Bacillus subtilis shuttle vector

PHY300PLK is one of the smallest hybrids of a new series of chimeric plasmids using the parental plasmids, pACYC177 of E.coli and pAM-alpha of Streptococcus faecalis. This shuttle vector for E.coli and B.subtilis contains an RNA primer gene for ori-177; the R-Tc gene; the R-Ap gene; Rep-alpha-1 gene, which is the plasmid replication gene; two replication origins, one for E.coli and the other for B. subtilis; and a polylinker. In the project of iGEM22_ZJU-China, we utilize this plasmid for the protein expression in Bacillus subtilis.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal XbaI site found at 6
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal BglII site found at 11
  Illegal BamHI site found at 27
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal XbaI site found at 6
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 37
  Illegal XbaI site found at 6
  Illegal PstI site found at 15
  Illegal PstI site found at 3830
  Illegal AgeI site found at 4709
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 2587
  Illegal BsaI site found at 4004