Composite

Part:BBa_K4201016

Designed by: David Birkhaeuser   Group: iGEM22_CU-Boulder   (2022-10-12)

Crte-cytoTDS-MBP_RUBY

The Crte-cytoTDS-MBP construct contains several genes. CrtE is the gene which encodes geranylgeranyl diphosphate synthase, an essential enzyme that synthesizes GGPP1. Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP2. The design of the Maltose binding protein coding sequence attached to the 3’ end of the TDS coding region is intended to assist with protein solubility in the cytosol3. immediately upstream of the the Crte-cytoTDS-MBP construct is a Gmubi promoter, and downstream of the construct is an AtHSP terminator. The RUBY marker, which is used to visualize gene transformation4, is also flanked by Gmubi and AtHSP respectively.



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 790
    Illegal BglII site found at 1618
    Illegal BglII site found at 3156
    Illegal BglII site found at 4203
    Illegal BglII site found at 5582
    Illegal BglII site found at 6517
    Illegal BglII site found at 10419
    Illegal BamHI site found at 5254
    Illegal BamHI site found at 9068
    Illegal XhoI site found at 2579
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1002
    Illegal PstI site found at 4800
    Illegal PstI site found at 5648
    Illegal PstI site found at 10856
    Illegal NgoMIV site found at 7004
    Illegal NgoMIV site found at 7583
    Illegal NgoMIV site found at 9296
    Illegal AgeI site found at 2321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 5448
    Illegal BsaI site found at 5728
    Illegal BsaI site found at 6675
    Illegal BsaI site found at 10656
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 5434
    Illegal BsaI.rc site found at 5714
    Illegal BsaI.rc site found at 6661
    Illegal BsaI.rc site found at 10642
    Illegal BsaI.rc site found at 10922
    Illegal SapI.rc site found at 3377
    Illegal SapI.rc site found at 3583
[edit]
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Parameters
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