Part:BBa_K4201011

Green Fluorescent Protein

Green fluorescent protein (GFP) is a commonly used reporter construct throughout the field of biology. When excited by UV or blue light, GFP will fluoresce green, making it an excellent reporter construct¹. GFP allows for the survival of the genetically modified plant unlike a GUS reporter, which is lethal to the plant².
When observing the soybeans with the GFP reporter under UV light, green fluorescence was observed in both the transgenic soybeans and the control without transgenes. Because of this, a GFP reporter was not a good reporter to use in our soybean constructs. This is due to autofluorescence from excitement of molecules that are naturally occurring in plants by UV light. The most common of these molecules are chlorophyll and lignin³.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 228
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 641
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 228
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 228
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 737