Coding

Part:BBa_K4201008

Designed by: Maya Li Nelson   Group: iGEM22_CU-Boulder   (2022-10-01)


TAT

Taxadiene-5α-ol-O-acetyl Transferase (TAT) is an acetyl transferase that acetylates Taxadiene-5α-ol into Taxadiene-5α-yl-acetate which is the third step in the synthesis pathway of paclitaxel1. The presence of this enzyme is very important for the biosynthesis of paclitaxel2.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 759
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 759
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 759
    Illegal BglII site found at 226
    Illegal BglII site found at 428
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 759
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 759
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1337


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Categories
Parameters
None