Coding
CrtE

Part:BBa_K4201003

Designed by: Maya Li Nelson,   Group: iGEM22_CU-Boulder   (2022-10-01)


CrtE

CrtE is the gene which encodes geranylgeranyl diphosphate synthase, an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized1. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway2. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring additional export tags.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 750
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 926


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Categories
Parameters
None