Coding

Part:BBa_K4201001

Designed by: Maya Li Nelson   Group: iGEM22_CU-Boulder   (2022-10-01)


Tc.cytoGGPPS

Geranylgeranyl diphosphate synthase is an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway1. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring us to add export tags. The amino acid sequence for this protein was run through a codon optimization program provided for maximum efficacy in soybeans.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 42
    Illegal BglII site found at 971
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 961
    Illegal NgoMIV site found at 372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1148


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Categories
Parameters
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