Composite

Part:BBa_K4199009

Designed by: Yongzhu Li   Group: iGEM22_NWU-CHINA-A   (2022-10-09)


J23119_Promoter-RBS-ChuA-RBS-HrtR-B0025_Terminator

It is used to regulate pigment expression according to heme level. Heme enters the cell via chuA-encoded proteins and binds to expressed HmuO, thereby inhibiting the HrtO promoter. And it can be expressed in E. coli Nissle1917(EcN).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2341
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2341
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2341
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None