Composite
Part:BBa_K4199009
Designed by: Yongzhu Li Group: iGEM22_NWU-CHINA-A (2022-10-09)
J23119_Promoter-RBS-ChuA-RBS-HrtR-B0025_Terminator
It is used to regulate pigment expression according to heme level. Heme enters the cell via chuA-encoded proteins and binds to expressed HmuO, thereby inhibiting the HrtO promoter. And it can be expressed in E. coli Nissle1917(EcN).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2341
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2341
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2341
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |