Composite

Part:BBa_K4199007

Designed by: Yongzhu Li   Group: iGEM22_NWU-CHINA-A   (2022-10-08)


T7_Promoter-RBS- ChuA-RBS-HrtR-T7_Terminator

Heme is transferred into cells and binds to expressed HmuO to release inhibition of HrtO promoter. It can be expressed in Escherichia coli BL21. Build with BBa_K4199006 and BBa_K4199007.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2654
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2325
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None