Part:BBa_K4194007

EGFP（+LVA）

a strong reporter. EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

Ex:488nm Em :507nm EC :55,900(M-1 cm-1) QY:0.6 Brightness:33.54 pKa:6.0 Maturation:25.0min Lifetime:2.6ns

A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.

ssrA-LVA tag

In E. coli [wild-type (sspB+ clpX+)], the adaptor sspB binds ssrAtagged substrates to ClpXP proteases, resulting in a modest increase in their degradation rate.

LVA tag is commonly found attached to repressors in various gene networks (e.g. oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB. ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

color	green
emission	507
excitation	488
tag	ssrA-LVA

The bacteria solution is green because of the expression of EGFP.