Coding

Part:BBa_K4194006

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


δ activator(+LVA)

The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ssrA-LVA tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 79
    Illegal NgoMIV site found at 174
    Illegal NgoMIV site found at 189
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None