Coding

Part:BBa_K4194004

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


pleD(+LVA)

pleD gene of bicyclic acid ester cyclase (DGC)+LVA. The intracellular c-di-GMP concentration is regulated by two enzymes with opposite functions, namely bicyclic acid ester cyclase (DGC) which synthesizes c-di-GMP and phosphodiesterase (PDE) which degrades c-di-GMP. A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 458
    Illegal NotI site found at 607
    Illegal NotI site found at 710
    Illegal NotI site found at 1357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None