Coding

Part:BBa_K4194002

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


LacI-LVA

Coding region for the LacI protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 913
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 913
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 913
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 913
    Illegal NgoMIV site found at 429
    Illegal AgeI site found at 6
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None