DNA

Part:BBa_K4194001

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


c-di-GMP riboswitch

Ribose switch is a RNA sequence in the 5'-untranslated region (5'-UTR) of mRNA, which includes the aptamer domain (AD), a conserved sequence that can recognize and bind to ligands, and the expression platform (EP), which has variable structure and can regulate the downstream coding genes. The c-di-GMP riboswitch may form a terminator structure, which shuts down the transcription of downstream genes without c-di-GMP. However, in the presence of c-di-GMP, no terminator will be formed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 67


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Categories
Parameters
None