Coding

Part:BBa_K419008

Designed by: YANG TUNG CHIN   Group: iGEM10_TzuChiU_Formosa   (2010-10-19)

alkG with rbs

alkG is rubredoxin coding gene from alkB1GHJ operon of a hydrocarbone-degrating marine bacterium, Alcanivorax Borkumensis SK2. When A. Borkumensis SK2 doing oil degradation, alkG provide electron to alkB1, and let alkB1 turn long chain alkane of crude oil into alkanol. We construct alkG with RBS (BBa_B0034). This part is provided by project: [http://2010.igem.org/Team:TzuChiU_Formosa/Poseidon Poseidon- save the world].


NCBI GENE ID: 4213398


Reference

Schneiker, et al. Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. Nature Biotechnology 24 (2006).

MM Yakimov, et al. Obligate oil-degrading marine bacteria. Curr Opin Biotechnol (2007 Jun).

JS Sabirova, et al. Proteomic Insights into Metabolic Adaptation in Alcanivorax borkumensis Induced by Alkane Utilization.Journal of Bacteriology (2006 Jun).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 370


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