Plasmid

Part:BBa_K4190024

Designed by: Zoe L. Petroff   Group: iGEM22_UCSC   (2022-09-22)


pET28:GLP1R

His-tagged GLP-1 R binding site expression system in E. coli. See parts BBa_K4190000 and BBa_K4190020.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 616
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 682
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 616
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 616
    Illegal NgoMIV site found at 714
    Illegal NgoMIV site found at 2302
    Illegal NgoMIV site found at 2462
    Illegal NgoMIV site found at 5509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3382
[edit]
Categories
Parameters
None