Plasmid

Part:BBa_K4190023

Designed by: Zoe L. Petroff, Neo Peng   Group: iGEM22_UCSC   (2022-09-22)


mg-int-trp1-hyg_1432-4a:Ex4

His-tagged Exendin-4 expression system with integration at S. cerevisiae tryptophan homology sites within the genome. See parts BBa_K4190010, BBa_K4190011, BBa_K4190013, and BBa_K4190019.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1469
    Illegal PstI site found at 1478
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 3263
    Illegal SpeI site found at 1469
    Illegal PstI site found at 1478
    Illegal NotI site found at 3554
    Illegal NotI site found at 5317
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 707
    Illegal XhoI site found at 1059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1469
    Illegal PstI site found at 1478
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1469
    Illegal PstI site found at 1478
    Illegal NgoMIV site found at 1394
    Illegal NgoMIV site found at 3293
    Illegal AgeI site found at 323
    Illegal AgeI site found at 2212
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 827
[edit]
Categories
Parameters
None