Plasmid
Part:BBa_K4190022
Designed by: Zoe L. Petroff, Neo Peng Group: iGEM22_UCSC (2022-09-22)
mg-Int-leu2-kan_1420:Ex4
His-tagged Exendin-4 expression system with integration at S. cerevisiae Leucine homology sites within the genome. See parts BBa_K4190010, BBa_K4190011, BBa_K4190012, and BBa_K4190019.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 10
Illegal SpeI site found at 1469
Illegal PstI site found at 1478 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 1469
Illegal PstI site found at 1478
Illegal NotI site found at 3401
Illegal NotI site found at 5164 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BglII site found at 707
Illegal XhoI site found at 1059 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal XbaI site found at 10
Illegal SpeI site found at 1469
Illegal PstI site found at 1478 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal XbaI site found at 10
Illegal SpeI site found at 1469
Illegal PstI site found at 1478
Illegal NgoMIV site found at 1394
Illegal NgoMIV site found at 5494
Illegal AgeI site found at 323 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 827
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Categories
Parameters
None |