Plasmid

Part:BBa_K4190021

Designed by: Zoe L. Petroff   Group: iGEM22_UCSC   (2022-09-22)


pET28:Ex4

His-tagged Exendin-4 expression system in E. coli. See parts BBa_K4190000 and BBa_K4190018.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 361
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 231
    Illegal BglII site found at 427
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 361
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 361
    Illegal NgoMIV site found at 459
    Illegal NgoMIV site found at 2047
    Illegal NgoMIV site found at 2207
    Illegal NgoMIV site found at 5254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3127


[edit]
Categories
Parameters
None